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Prof. Li-Chu Tsai



Dr. Li-Chu Tsai

Research Laboratory

Biomolecular Engineering Laboratory

Educational Background

Gothenburg University, Sweden

Chemistry, Ph. D.

National Cheng Kung University, Tainan

Chemistry, B.S.


Molecular Science Building, 2nd floor 

Tel.: +886-2-27712171 ext. 2415

Research Laboratory

Molecular Science Building, 4th floor, Biomolecular Engineering Laboratory

Tel: +886-2-27712171 ext. 2436

E mail




1.    2011~ Professor, Department of Molecular Science and Engineering, National Taipei University of Technology

2.     1996/12~ 2003/2, Postdoctor research fellow, Institute of Molecular Biology, Academia Sinica, Taiwan


Research Specialty

1.   Crystal Structure of Glycoside hydrolase (GH 6, 16, and 48 Enzymes)

Enzyme–carbohydrate interactions and selective hydrolysis of glycosidic bonds are crucial for cellular processes, energy uptake, and degradation. Glycosyl hydrolases have the ability to recognize and bind particular carbohydrates and then cleave the specific glycosidic bond between either two or more carbohydrates or between a carbohydrate and a non-carbohydrate moiety. We have determined three family enzymatic structures with their substrate(product), a mix b/a GH6, a jellyroll b- of GH 6, and a (a/a) GH48.

2.  Biomolecule detection

Nanowire field-effect-transistor (NWFET) based sensors are shown to possess the advantage as a label-free, highly sensitive and real-time means of detection, making it an excellent candidate for the detection of the biologically active molecular interactions. Using Si-NWFETs made by state-of-the-art microfabrication technology, we have demonstrated the capacity of the NWFET based sensors to monitor the DNA hybridizations with a record-high sensitivity (0.1fM). Furthermore, we demonstrated the following applications of this device in the detections of E coli, H1N1 virus, and gaseous substances such as ethanol and methanol.


Publication (2010~2016)


  1. Li-Chu Tsai, Hao-Ying Hsieh, Kun-Ying Lu, Sin-Yu Wang  and Fwu-Long Mi*“EGCG/gelatin-doxorubicin gold nanoparticles enhance therapeutic efficacy of doxorubicin for prostate cancer treatment” Nanomedicne. (2016). 11(1), 9-30 (IF~6.2, R/C=12/123, Medicine, Research & Experimental)
  2. Li-Chu Tsai*, Imamaddin Amiraslanov, Hung-Ren Chen, Yun-Wen Chen, Hsiao-Lin Lee, Po-Huang Liang, and Yen-Chywan Liaw*“Crystal structures of exoglucanase from Clostridium cellulovorans: cellotetraose binding and cleavage” Acta Cryst. (2015). F71, 1264–1272  (IF~0.524, R/C=17/23, Crystallography)
  3. Yo-Chia Chen, Wan-Ting Chen, Jeng-Chen Liu, Li-Chu Tsai, Hsueh-Ling Cheng* “A highly active beta-glucanase from a new strain of rumen fungus Orpinomyces sp.Y102 exhibits cellobiohydrolase and cellotriohydrolase activities ”  Bioresource Technology (2014), 170,513-521. (IF~=4.98, R/C=20/158, Biotehnology & Applied Microbiology)
  4. Chia-Jung Chu, Chia-Sen Yeh, Chun-Kai Liao, Li-Chu Tsai*, Chun-Ming HuangHung-Yi Lin*, Jing-Jong Shyue, Yit-Tsong Chen, and Chii-Dong Chen*“Improving Nanowire Sensing Capability by Electrical Field Alignment of Surface Probing Molecules” Nano Letter (2013), 13, 2564-2569 (IF~13.025, R/C=8/239, Materials science, Multidisciplinary)
  5. Yo-Chia Chen, Yu-Chuan Chiang, Fu-Yuan Hsu, Li-Chu Tsai*, Hsueh-Ling Cheng* “Structural modeling and further improvement in pH stability and activity of a highly-active xylanase from an uncultured rumen fungus”Bioresource Technology (2012), 123,125-134.(IF~=4.98, R/C=20/158, Biotehnology & Applied Microbiology)
  6. Chih-Heng Lin, Chia-Jung Chu, Kang-Ning Teng, Yi-Jr Su, Chii-Dong Chen, Li-Chu Tsai, and Yuh-Shyong Yang “Recovery Based Nanowire Field-Effect Transistor Detection of Pathogenic Avian Influenza DNA”Japanese Journal of Applied Physics 51 (2012) 02BL02-1-5(IF~1.08, R/C=76/125, Physics, Applied)
  7. Tsai, L. C.*, Hsiao, C. H., Liu, W. Y., Li-Ming Yin, L. M., and Shyur, L. F.  “Structural basis for the inhibition of 1,3-1,4--D-glucanase by noncompetitive calcium ion and competitive Tris inhibitors”Biochemical and Biophysical Research Communications (2011), 407, 953-958.(IF~2.48, R/C=173/290, Biochemistry & Molecular biology)
  8. Chen, J-H., Tsai, L. C.*, Huang, H.-C., and Shyur, L. F. “Structural and catalytic roles of amino acid residues located at substrate binding pocket in Fibrobacter succinogenes 1,3-1,4--D-glucanase”Proteins: structure, function, and bioinformatics (2010), 78, 2820-2830.(IF~3.39, R/C=108/290, Biochemistry & Molecular biology)
  9. Tsai, L. C.*, Cheng, I. C., Tu, M.C., Chen, C. D. and Lin, H. Y. “Formation of single-electron-transistors using self-assembly of nanoparticle chains”Journal of Nanoparticle Research (2010), 12, 2859-2864. (IF~ 3.25, R/C= 24/66, Nanoscience & Nanotechnology)




1.      “Functional and structural studies of carbohydrate-binding modules”

National Science Council 2009/7-2012/8. 


2.      Structural and functional studies of OsCel6 cellobiohydrolase”,

National Science Council 2012/8-2013/7. 


3.      “Silicon-nanowire devices for high sensitive biomolecule detection”

National Science Council 2015/8-2016/7.